FM drop out mixture, minus Arginine and w/o Cysteine, 7400 mg/l Size:100g 13 pH (100 g/l) 3

13 pH (100 g/l) 3

The pH (and pKa at ionic strength I≠0) of the buffer solution changes with concentration and temperature

Tryptone 10 Yeast extract 5 Agar 15 Final pH 7

Chemical formula C4H9NO3 Solubility (20° C) 90 g/l Mol

Suspend 11

FM drop out mixture, minus Arginine and w/o Cysteine, 7400 mg/l Size:100g 13 pH (100 g/l) 3The FM drop out mixture, minus Arginine and without Cysteine, is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many Drop Out mixtures to select for auxotrophic requirements and transformants. Each FM Drop Out mixture contain all components except for one or two essential Amino acids, i. e. the dropped out supplements. FM Amino acids drop out mixtures are used in combination with FM Minimal medium w o Amino