FM Minimal medium w/o Cysteine and w/o Methionine Size:1000g These buffers were developed with

These buffers were developed with the following criteria in mind: midrange pKa

Assay (HPLC) ≥99% Melting range 148

Chemical formula

6 (20 mN) Tryptone 20 Yeast extract 5 NaCl 0

Soluble in water

FM Minimal medium w/o Cysteine and w/o Methionine Size:1000g These buffers were developed withThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to