SIH Medium Size:1kg Cas Number: 001132-61-2

Cas Number: 001132-61-2

the “dropped out” supplement

This formulation supports the growth of most strains that are capable of growing on HL5

2 K2HPO4 9

2 at 25°C Suspend 28 gram powdered medium in 1 litre distilled water

SIH Medium Size:1kg Cas Number: 001132-61-2SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic