Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g 5 Na2HPO4 0

5 Na2HPO4 0

Biotin is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin

0 Beef Extract 5

in the 1960s

Final concentration is 40mM Tris Base

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g 5 Na2HPO4 0Boric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room